Association of gene polymorphism methylenetetrahydrofolate reductase cytosine-to-thymidine substitution at nucleotide 677 with cardiovascular and metabolic risk in morbidly obese patients

    Authors

    Keywords

    methylenetetrahydrofolate reductase, hypertension, diabetes

    DOI

    https://doi.org/10.15836/ccar2019.235

    Full Text

    Introduction : Region near the gene encoding methylenetetrahydrofolate reductase (MTHFR) is among eight loci associated with blood pressure. ( 1 - 3 ) The aim of this study is to show connection between polymorphism of MTHFR C667T and hypertension, diabetes, prediabetes and obstructive sleep apnea in obese Croatian patients. Patients and Methods : We included 88 patients from a multidisciplinary weight management program in which genetic analysis on MTHFR gene polymorphism was tested. Patients were divided in 3 groups: 36 patients with MTHFR C677T healthy genotype CC (27 women, 9 men; age 46.4±10.1 year; BMI 44.9±8.8 kg/m 2 ), 38 patients with MTHFR C677T heterozygous mutation CT (27 women, 11 men; age 46.9±11.4 year; BMI 44.6±8.6 kg/m 2 ), 14 patients with MTHFR C677T homozygous mutation TT (12 women, 2 men; age 50.1±15.5 year; BMI 40.2±6.9 kg/m 2 ). In each group the incidence of hypertension, prediabetes, diabetes and obstructive sleep apnea (OSA) was determined. Results : Patients with genetic mutation MTHFR C677T:CT ( Figure 1 ) had the highest incidence of arterial hypertension (65.8%), diabetes (18.4%), prediabetes (18.4%) and OSA (31.6%) with the highest average apnea hypopnea index (AHI) of 17.3±24.7, even 13.2% of patients used continuous positive airway pressure (CPAP). Patients with healthy genotype MTHFR C677T:CC had lower incidence of arterial hypertension (44.4%), prediabetes (11.1%), diabetes (13.9%), OSA (25%), average AHI 11.3±15.9 and only 8% of patients used CPAP. Patients with MTHFR C677T:TT polymorphism had the lowest arterial hypertension incidence (42.9%), the highest prediabetes incidence (42.9%), middle OSA prevalence (28.6%), AHI 7.7±7.6. The graph shows hypertension, prediabetes and obstructive sleep apnea distribution in the group of patients with methylenetetrahydrofolate reductase cytosine-to-thymidine substitution at nucleotide 677 healthy genotype CC, heterozygous mutation CT and homozygous mutation TT. MTHFR C677T:CC - methylenetetrahydrofolate reductase cytosine-to-thymidine substitution at nucleotide 677 healthy genotype CC; MTHFR C677T:CT - methylenetetrahydrofolate reductase cytosine-to-thymidine substitution at nucleotide 677 heterozygous mutation CT; MTHFR C677T:TT - methylenetetrahydrofolate reductase cytosine-to-thymidine substitution at nucleotide 677 homozygous mutation TT. Conclusion : MTHFR C677T:CT polymorphism is the most common gene polymorphism in our group of morbidly obese patients. MTHFR C677T:CT polymorphism compared to MTHFR C677T:CC and MTHFR C677T:TT polymorphisms carries the highest risk for arterial hypertension, metabolic disorders (diabetes) and obstructive sleep apnea. Homozygotes MTHFR C677T:TT carries the highest risk for prediabetes. Further investigation is needed to explore this correlation.

    Cardiologia Croatica
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    Association of gene polymorphism methylenetetrahydrofolate reductase cytosine-to-thymidine substitution at nucleotide 677 with cardiovascular and metabolic risk in morbidly obese patients

    Extended Abstract
    Issue9-10
    Published
    Pages235
    PDF via DOIhttps://doi.org/10.15836/ccar2019.235
    methylenetetrahydrofolate reductase
    hypertension
    diabetes

    Authors

    Rea Levicki*ORCIDCroatia
    Juraj JugORCIDUniversity of Zagreb School of Medicine, Zagreb, Croatia
    Ines VinkovićORCIDUniversity of Zagreb School of Medicine, Zagreb, Croatia
    Filip MustačORCIDUniversity of Zagreb School of Medicine, Zagreb, Croatia
    Martina MatovinovićORCIDUniversity Hospital Centre Zagreb, Zagreb, Croatia
    Lada BradićORCIDUniversity Hospital Centre Zagreb, Zagreb, Croatia
    Jadranka SertićUniversity Hospital Centre Zagreb, Zagreb, Croatia
    Martina Lovrić BenčićORCIDUniversity Hospital Centre Zagreb, Zagreb, Croatia

    Full Text

    Introduction : Region near the gene encoding methylenetetrahydrofolate reductase (MTHFR) is among eight loci associated with blood pressure. ( 1 - 3 ) The aim of this study is to show connection between polymorphism of MTHFR C667T and hypertension, diabetes, prediabetes and obstructive sleep apnea in obese Croatian patients. Patients and Methods : We included 88 patients from a multidisciplinary weight management program in which genetic analysis on MTHFR gene polymorphism was tested. Patients were divided in 3 groups: 36 patients with MTHFR C677T healthy genotype CC (27 women, 9 men; age 46.4±10.1 year; BMI 44.9±8.8 kg/m 2 ), 38 patients with MTHFR C677T heterozygous mutation CT (27 women, 11 men; age 46.9±11.4 year; BMI 44.6±8.6 kg/m 2 ), 14 patients with MTHFR C677T homozygous mutation TT (12 women, 2 men; age 50.1±15.5 year; BMI 40.2±6.9 kg/m 2 ). In each group the incidence of hypertension, prediabetes, diabetes and obstructive sleep apnea (OSA) was determined. Results : Patients with genetic mutation MTHFR C677T:CT ( Figure 1 ) had the highest incidence of arterial hypertension (65.8%), diabetes (18.4%), prediabetes (18.4%) and OSA (31.6%) with the highest average apnea hypopnea index (AHI) of 17.3±24.7, even 13.2% of patients used continuous positive airway pressure (CPAP). Patients with healthy genotype MTHFR C677T:CC had lower incidence of arterial hypertension (44.4%), prediabetes (11.1%), diabetes (13.9%), OSA (25%), average AHI 11.3±15.9 and only 8% of patients used CPAP. Patients with MTHFR C677T:TT polymorphism had the lowest arterial hypertension incidence (42.9%), the highest prediabetes incidence (42.9%), middle OSA prevalence (28.6%), AHI 7.7±7.6. The graph shows hypertension, prediabetes and obstructive sleep apnea distribution in the group of patients with methylenetetrahydrofolate reductase cytosine-to-thymidine substitution at nucleotide 677 healthy genotype CC, heterozygous mutation CT and homozygous mutation TT. MTHFR C677T:CC - methylenetetrahydrofolate reductase cytosine-to-thymidine substitution at nucleotide 677 healthy genotype CC; MTHFR C677T:CT - methylenetetrahydrofolate reductase cytosine-to-thymidine substitution at nucleotide 677 heterozygous mutation CT; MTHFR C677T:TT - methylenetetrahydrofolate reductase cytosine-to-thymidine substitution at nucleotide 677 homozygous mutation TT. Conclusion : MTHFR C677T:CT polymorphism is the most common gene polymorphism in our group of morbidly obese patients. MTHFR C677T:CT polymorphism compared to MTHFR C677T:CC and MTHFR C677T:TT polymorphisms carries the highest risk for arterial hypertension, metabolic disorders (diabetes) and obstructive sleep apnea. Homozygotes MTHFR C677T:TT carries the highest risk for prediabetes. Further investigation is needed to explore this correlation.